English  |  正體中文  |  简体中文  |  Items with full text/Total items : 1592/1691
Visitors : 1423133      Online Users : 259
RC Version 5.0 © Powered By DSPACE, MIT. Enhanced by NTU Library IR team.
Scope Adv. Search
LoginUploadHelpAboutAdminister

Please use this identifier to cite or link to this item: http://140.112.115.32:8080/ir/handle/987654321/1915

Title: Abnormally low expression of connexin 37 and connexin 43 in subcutaneously transplanted cryopreserved mouse ovarian tissue
Authors: Robert Kuo-Kuang Lee;Sheng-Hsiang Li;Chung-Hao Lu;Hsin-Yi Ho;Ying-Jie Chen;Hung-I Yeh
Contributors: 醫學系
Date: 2008-10-01
Issue Date: 2015-07-01 13:40:18 (UTC+8)
Abstract: ● Purpose: To analyze the gap junction proteins connexin 37 (Cx37) and connexin 43 (Cx43) after subcutaneous transplantation of cryopreserved mouse ovarian tissue.
● Methods: Expression of gap junction genes was assessed by immunohistochemistry and real-time polymerase chain reaction (PCR) in transplanted cryopreserved ovarian tissue compared with that of normal ovarian tissue. Apoptosis of ovarian cells was evaluated by using the terminal deoxynucleotidyl transferase-mediated biotinylated deoxyuridine triphosphates nick end-labeling method.
● Results: After subcutaneous transplantation, Cx37 and Cx43 mRNA and protein expression were significantly lower in cryopreserved than in normal ovarian tissue. Apoptosis was increased in granulosa cells from antral follicles of the cryopreserved tissue.
● Conclusion: After cryopreservation and subcutaneous transplantation of ovarian tissue, proteins forming gap junctions between oocytes and granulosa cells are under-expressed compared with normal controls.
Relation: Journal of Assisted Reproduction and Genetics, 25(9-10), 489-497. https://doi.org/10.1007/s10815-008-9264-8
Appears in Collections:[醫學系] 期刊論文

Files in This Item:

File Description SizeFormat
index.html(DOI)0KbHTML18View/Open


All items in MMCIR are protected by copyright, with all rights reserved.

 


DSpace Software Copyright © 2002-2004  MIT &  Hewlett-Packard  /   Enhanced by   NTU Library IR team Copyright ©   - Feedback